thioglycollate broth medium  (Beijing Solarbio Science)

Journal: Dentistry Journal

Article Title: Synergistic Effect of Metronidazole and Chlorhexidine against Porphyromonas gingivalis Growth: An In Vitro Study

doi: 10.3390/dj12100307

Figure Lengend Snippet: ( A – C ) Effect of MTZ and CHX alone or in combination against P. gingivalis growth. Control samples were grown in thioglycollate broth without any supplementation (ctr), while compounds were tested alone at different concentrations, ranging from 1 mg/mL to 0.015 mg/mL for MTZ ( A ), from 1 mg/mL to 0.03 mg/mL for CHX ( B ), or in combination with 0.016 mg/mL MTZ plus 0.03–0.06 mg/mL CHX ( C ). Each absorbance value (OD 450) is mean ± SD of three replicates. One representative of three independent experiments is shown. Means were compared by using Student’s t -test. Significance level for p values was considered as ** p < 0.01; and *** p < 0.001.

Article Snippet: Porphyromonas gingivalis (ATCC 33277) cells maintained as frozen stock were firstly grown in thioglycollate medium broth (Thermo Scientific, Ann Arbor, MI, USA) in an anaerobic atmosphere composed of 10% H 2 , 5% CO 2 , and 85% N 2 at 37 °C.

Techniques: Control